Jacob L. Steenwyk, Abigail L. Lind, Laure N.A. Ries, Thaila F. dos Reis, Lilian P. Silva, Fausto Almeida, Rafael W. Bastos, Thais Fernanda de Campos Fraga da Silva, Vania L.D. Bonato, André Moreira Pessoni, Fernando Rodrigues, Huzefa A. Raja, Sonja L. Knowles, Nicholas H. Oberlies, Katrien Lagrou, Gustavo H. Goldman, Antonis Rokas

 Six Clinical Isolates Previously Characterized as A. nidulans Are Diploid

To gain insights into the genetic diversity of clinical isolates of A. nidulans, we analyzed 7 isolates from patients with different pulmonary diseases and compared them to haploid (A4) and the laboratory-induced diploid (R21/R153) reference strains of A. nidulans (Table 1). Using microscopy-based and/or molecular biology methods, all 7 isolates had previously been identified as A. nidulans, all are similar in appearance when grown in standard laboratory conditions (Figure S1), and two were analyzed as A. nidulans isolates in previous experimental studies [

  • Lee M.J.
  • Liu H.
  • Barker B.M.
  • Snarr B.D.
  • Gravelat F.N.
  • Al Abdallah Q.
  • Gavino C.
  • Baistrocchi S.R.
  • Ostapska H.
  • Xiao T.
  • et al.
The fungal exopolysaccharide galactosaminogalactan mediates virulence by enhancing resistance to neutrophil extracellular traps.