Andre Arashiro Pulschen, Delyan R. Mutavchiev, Siân Culley, Kim Nadine Sebastian, Jacques Roubinet, Marc Roubinet, Gabriel Tarrason Risa, Marleen van Wolferen, Chantal Roubinet, Uwe Schmidt, Gautam Dey, Sonja-Verena Albers, Ricardo Henriques, Buzz Baum

Discussion

Here, we describe the development of the “Sulfoscope,” an imaging platform that makes it possible to image Sulfolobus cell divisions live. Our first use of the Sulfoscope revealed a tight coupling between DNA reorganization, nucleoid separation, and membrane deformation during division (Figure 2D). These events appear to occur in a defined order in the wild-type cell (both S. acidocaldarius and S. solfataricus; Figures S2A and S2B). This begins as replicated chromosomes lose their diffuse organization as they separate and is followed by a relatively sudden DNA compaction, which coincides with the onset of furrowing. Thus, DNA compaction may help to ensure that the segregated chromosomes remain out of the way of the furrow as it closes. These findings are in line with observations in fixed cells of Sulfolobus [

  • Popławski A.
  • Bernander R.
Nucleoid structure and distribution in thermophilic Archaea.